Molecular biology

Understanding of fundamental biological processes which determine the status of cellular genetic information.

Exposure to selected techniques used in molecular biology research.

Through the lectures students will get expertise on the genome organization and maintenance. Special emphasis will be given to processes which generate genetic variation. Students will also learn about the basic steps of gene expression and its regulation, including control through epigenetic mechanisms. Whenever possible, descriptions of the respective prokaryotic and eukaryotic processes will be confronted.

During the practicals students will learn how to isolate genomic DNA and how to use it for basic analytical purposes like blotting and amplification. This part will also cover basics of molecular cloning with the use of phage-derived vectors. Finally, students will get insights into computer-aided analysis of nucleotide sequence data.

Genomes

DNA replication

Gene expression – transcription and translation

Protein sorting and posttranslational protein modifications

Cell communication

Epigenetic phenomena

Mutations and DNA repair

DNA recombination

Isolation and restriction of plant genomic DNA

DNA electrophoresis and blotting

Polymerase chain reaction

Use of bacteriophage M13 vectors

DNA sequence analysis

Alberts B, Johnson A, Lewis J, Morgan D, Raff M, Roberts K, Walter P (2014) Molecular biology of the cell (6th edn). Garland Science

Lodish H, Berk A, Kaiser CA, Krieger M, Bretscher A, Ploegh H, Amon A, Scott MP (2012) Molecular cell biology (7th edn). Freeman, W. H. & Company

Russell PJ (2010) Genetics: a molecular approach (3rd edn). Pearson

EPB2_W01, EPB2_W02, EPB2_W07

EPB2_U01, EPB2_U03, EPB2_U06, EPB2_U11

EPB2_K01, EPB2_K02, EPB2_K05, EPB2_K06

Test 703