Resistance Plant Breeding
|Kod przedmiotu:||R.9sX.RPB.SM.ROSAY||Kod Erasmus / ISCED:||(brak danych) / (brak danych)|
|Nazwa przedmiotu:||Resistance Plant Breeding|
|Jednostka:||Katedra Hodowli Roślin i Nasiennictwa|
|Punkty ECTS i inne:||
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The course aims focus on introduction the basic knowledge about impact of diseases to global food production, plant-pathogen system interaction, various assessment methods of susceptibility to plant diseases cause by selected biotic factors (fungi, viruses, bacteria) and the possibilities of controlling plant diseases with alternative methods to chemical protection based on resistant or less susceptible varieties, created with conventional breeding method application, as well as generated through genetic engineering.
The importance of plant diseases in global food production, etiology of disease, the reactions of plants to infection. Concepts and mechanisms of plant resistance to disease. Molecular basis of plant resistance to disease. Genetic basis of breeding resistant varieties to disease. Biotechnological methods application in resistance plant breeding, methods of susceptibility assessment to facultative pathogens.
Detailed schedule of lectures:
1. Biotic stress caused by viruses, bacteria and fungi. Abiotic stress: cold, frost, drought, pollution. Plant pathogens and their impact on global food production - 2 h.
2. Plant disease symptoms, etiology of plant diseases -1 h.
3. Changes in plants caused by infection and disease progression. Physiological changes accompanying disease development (respiration, photosynthesis). Structural and anatomical changes -1 h.
4. Terms and plant defense mechanisms
a) resistance- susceptibility , scale
b) types of plant defense mechanisms
a) types of plant response to infection
Molecular basis of resistance to infection -2 h.
5. Genetic basis of resistance breeding, host – pathogen interaction -1 h.
6. Classical breeding methods in creation of resistant varieties
a) selection of homozygous lines
b) pedigree breeding
c) backcross method
Biotechnological methods in resistance plant breeding -3 h.
Detailed schedule of lab classes
1. Facultative pathogen propagation, inoculum production for lab, greenhouse and field tests purposes – media, conditions, preservation -2 h.
2. Evaluation of resistance in cereals to facultative pathogens e.g. Fusarium
a) laboratory test
b) greenhouse test
c) field test
Impact of Fusarium on food production (quantitative and qualitative loses) - 3h.
3. Calculation of disease severity and plant resistance based on lab, greenhouse and field tests. Estimation of seedling blight severity (5 degree scale, weight reduction), determining of phenolic acid concentration. Reduction of yield components in cereals. Species, varieties and lines variability of disease severity among selected cereals -4 h.
4. Marker assisted selection (MAS) in plant breeding (utilizing PCR-based markers in triticale and wheat selection) - 4 h.
5. Molecular diagnostics – detection of Fusarium infection and food contamination based on PCR method (species specific primers and trichotecene genes specific primers) -2 h.
1. Number of hours and ECTS points – obligatory course, hours: 25; ECTS 3
2. Number of hours and ECTS points – facultative course, hours: ; ECTS
3. Sum of hours and ECTS points, which student gains through face to face interaction with lecturer (lectures, lab classes, seminars). Hours: 25; ECTS 3,0
4. Sum of hours and ECTS points, which student gains through participation in practical part of course e.g. lab classes, projects, field trips, workshops. Hours: 15 ECTS; 2
5. Predictable investment of students individual work (without participation of lecturer or with the participation in the frame of consultation) necessary to fulfill all tasks of the course. Hours: 10; ECTS: 1,0
1. Arseniuk E., Góral T., Czembor H.J. 1993. Reaction of triticale, wheat, and rye accessions to graminaceous Fusarium spp. infection at the seedling and adult plant growth stages. Euphytica 70: 175-183.
2. Dhan Pal Singh, 1986: Breeding for resistance to disease and insect pest. Springer - Verlag
3. Jacobs Th., Parlevist J.E., 1993: Durability of disease resistance, Kluwer Academic Publishers
4. Chełkowski J. , Golka L. , Jakubowski P., 2002. Putative resistance genes of cereals: structure and expected function, J. Appl. Genet. 43(3), pp. 297-308
5. Vanderplank J.E., 1984:Disease resistance in plants, Academic Press, INC.
6. Vidhyasekaran P., 2002: Bacterial disease resistance in plants, Food Products Press ®
7. Warzecha T., Zieliński A., Skrzypek E., Wójtowicz T., Moś M., 2012. Effect of mechanical damage on vigor, physiological parameters, and susceptibility of oat (Avena sativa) to Fusarium culmorum infection. Phytoparasitica 40: 29-36. DOI 10.1007/s12600-011-0196-y
|Efekty uczenia się:||
The learning outcomes
1. Students can explain the basic concepts, methods and effects of plant breeding with more attention to resistance breeding process
2. Students can characterize sources of variability in plant breeding (plant genetic resources, mutants and transgenic plants) in resistance breeding
3. Students can name and describe the stages of breeding process (primary stages as collecting various genotypes, crossing and selection process) in order to obtain a new variety
4. Students can propose breeding method for a particular species of cultivated plant considering way of sexual reproduction (self and open-pollinated) nature of traits inheritance
5. Students can assess genotypes with laboratory and field tests application susceptibility of various cereal to facultative pathogens.
6. Students can apply direct (lab and filed tests) and indirect (physiological parameters) indexes to evaluated breeding plant material.
7. Student know how to propagate and preserve facultative pathogens in order to perform
8. Student understand the importance of plant genetic resources protection, naturally occurring in centers of origin and the genotypes produced by artificial selection, as a potential source of variation
9. Organize the work in a team to perform a specific task,
10. Students can recognize and appreciate the relationship between resistance plant breeding and biological improvement in crop production, understands the need continuous training resulting from technological progress (as modern biotechnological methods)
11. Can point out advantages of resistance plant breeding as method of plant protection resulting in less environment pollution
|Metody i kryteria oceniania:||
• An examination of different germplasm (DH lines, varieties) to biotic stress, working individually and in teams of two people
• Reports from lab and greenhouse resistance tests
• An essay focused on disease in selected crop
• Activity in the class discussion
Average grade based on scores obtained in the lab classes
written test exam (about 20 questions from the entire range of the subject with four response options), 0 or 1 pt. for each question
Grade E (2.0) Very low level of understanding , less than 40%
Grade D (3.0) Basic information about the lectures and lab practicals, 45-55%
Grade C (3.5) Ability to discuss any problem at the intermediatelevel 60%
Grade B (4.0) Good understanding of the subject, ability to ask about different issues, 70%
Grade B+ (4.5) Very good knowledge about the subject, student is interested in the subject and asks for more information, works on his own 85%
Grade A (5.0) Excellent understanding, full discussion, very good lab practicals more than 90%
Właścicielem praw autorskich jest Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie.