Introduction to genetic engineering
|Kod przedmiotu:||H.1s.FEZa.I.SM.HZOXZ||Kod Erasmus / ISCED:||(brak danych) / (brak danych)|
|Nazwa przedmiotu:||Introduction to genetic engineering|
|Jednostka:||Katedra Fizjologii i Endokrynologii Zwierząt|
|Punkty ECTS i inne:||2.00|
The aim of teaching: familiarization of master course students with the basic concepts of genetic engineering and modern molecular methods and laboratory techniques, i.e. a technique for gene cloning in vectors, Southern blot, Northern blot, PCR, RT-PCR, real-time PCR, and techniques used in animal transgensis.
The effect of education: understanding and knowledge of the basic concepts as well as genetic engineering techniques applicable in laboratory conditions for genetic recombination, introduction of DNA fragments into cells of another organism, gene and whole organism cloning. Knowledge of modern methods applied in gene expression.
Guide to basic concepts of genetic engineering. Structure of nucleic acids, and their physical and chemical properties.
DNA and RNA modifying enzymes: DNA and RNA polymerases, nucleases, enzymes modifying the ends of DNA fragments, DNA ligase.
Restriction enzymes, nomenclature, distribution and application in genetic engineering.
Vectors - application in molecular cloning and transgensis.
Methods of nucleic acid analysis: Southern blot, Northern blot and slot-blot
PCR method - varieties and the application in laboratory work.
RT-PCR, Real-time PCR, siRNA – application in determination of gene expression.
1. T.A. Brown, „Genmes”, PWN, Warszawa, 2009.
2. “Molecular cloning: a laboratory manual (Sec. Ed.), J. Sambrook, E.F. Fritch i T. Maniatis, J. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989.
3. “Recombinant DNA”, James Watson i inn., Scientific American Books, New York, 1992.
4. „Genes V”, Benjamin Lewin, Oxford University Press, Oxford New York Tokyo, 1994.
|Efekty uczenia się:||
The student knows the most important scientific discoveries that have contributed to the development of genetic engineering and describes the structure and function of nucleic acids and explains the cellular processes of DNA and RNA. The student enumerates and characterizes each group of enzymes used in the manipulation of DNA and RNA, explains the importance of restriction enzymes in the laboratory techniques. He/she describes methods for DNA cloning in different types of vectors and explains application of DNA cloning techniques in genetic engineering. The student characterizes the different methods used in the analysis of DNA and RNA; explains ways of using known analytical methods in molecular experiments.
He/she knows how to use the knowledge of the structure and function of nucleic acids; The student can explain the meaning of restriction enzymes and can use them in DNA cloning; can prepare the experiment, the purpose of which is to introduce the gene into a vector, followed by its amplification in E. coli. He/she uses and selects the appropriate genetic engineering techniques for the analysis of nucleic acids. He/she is able to perform an experiment using PCR techniques, RT-PCR, qPCR; interprets the results of the analysis of gene expression.
|Metody i kryteria oceniania:||
Lectures: Time limited written exam.
Labs: Demonstration of practical skills.
The percentage scale for assessing educational results is defined as follows:
1. Fail (F): some more work required before the credit can be awarded; it is issued when during the course the student have obtained less than 55% of the required effects of at least from one of the three components of the educational results (K, S, SC)
2. Sufficient (E): performance meets the minimum criteria, it is issued when during the course the student have obtained at least 55% of the required effects of each components of the educational results (K, S, SC)
3. Satisfactory (D): fair but with significant shortcomings; it is issued on the basis of the arithmetic average of the three components of educational results (K, S, SC)
4. Good (C): generally sound work with a number of notable errors; it is issued on the basis of the arithmetic average of the three components of educational results (K, S, SC)
5. Very good (B): above the average standard but with some errors; it is issued on the basis of the arithmetic average of the three components of educational results (K, S, SC)
6. Excellent (A): outstanding performance with only minor errors; it is issued on the basis of the arithmetic average of the three components of educational results (K, S, SC)
Zajęcia w cyklu "Semestr letni 2019/2020" (zakończony)
|Okres:||2020-02-24 - 2020-09-30||
zobacz plan zajęć
Wykład, 15 godzin więcej informacji
|Prowadzący grup:||(brak danych)|
|Lista studentów:||(nie masz dostępu)|
|Zaliczenie:||Zaliczenie na ocenę|
Właścicielem praw autorskich jest Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie.